Composite

Part:BBa_K3747141

Designed by: Sophieke Lems   Group: iGEM21_Wageningen_UR   (2021-10-21)


ApbE - blue (type 1) copper protein 1 - blue (type 1) copper protein 2 - c550 Pseudomonas

Nitrous oxide reductase (Nos) is the last enzyme of the denitrification pathway and reduces nitrous oxide (N2O) into dinitrogen gas (N2). To express this enzyme in vivo, suitable electron donors are needed for Nos. This part contains possible electron donors (blue (type 1) copper proteins and cytochrome c550). Additionally, ApbE is needed for the activity of NosR.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1011
    Illegal EcoRI site found at 1326
    Illegal PstI site found at 210
    Illegal PstI site found at 510
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1011
    Illegal EcoRI site found at 1326
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 210
    Illegal PstI site found at 510
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1011
    Illegal EcoRI site found at 1326
    Illegal BamHI site found at 833
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1011
    Illegal EcoRI site found at 1326
    Illegal PstI site found at 210
    Illegal PstI site found at 510
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1011
    Illegal EcoRI site found at 1326
    Illegal PstI site found at 210
    Illegal PstI site found at 510
    Illegal NgoMIV site found at 1152
    Illegal NgoMIV site found at 1685
    Illegal NgoMIV site found at 1845
    Illegal NgoMIV site found at 2067
    Illegal AgeI site found at 843
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None